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Using CRISPR/Cas platform for Genetic Modification of Commercial Saccharomyces cerevisiae strains

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Abstract

Traditional wine fermentation is a complex microbial process initiated by various yeast species classified as Saccharomyces and non-Saccharomyces species.To better understand the different interactions occurring within wine fermentations and track a specific yeast population, we wish to obtain GFP-tagged yeast cells that stably expres fluorescence signal without compromising the fermentative capability of the strain.To this end, the CRISPR/Cas system was investigated to genetically modify the commercial Saccharomyces Saccharomyces cerevisiae diploid strain Lalvin EC 1118
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Dates and versions

hal-02466806 , version 1 (04-02-2020)

Identifiers

  • HAL Id : hal-02466806 , version 1

Cite

C. Eicher, Géraldine L. Klein, Pascale Winckler, Jean-Luc Parrou, Hervé Alexandre, et al.. Using CRISPR/Cas platform for Genetic Modification of Commercial Saccharomyces cerevisiae strains. OENO-IVAS 2019, Jun 2019, Bordeaux, France. ⟨hal-02466806⟩
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