Improving acid tolerance in Oenococcus oeni by Adaptive Evolution
Résumé
Oenococcus oeni is a lactic acid bacterium (LAB) mainly responsible for the malolactic fermentation (MLF) in
wine. MLF plays an important role in determining the final quality of wines
[1]. Even though this LAB is naturally present in musts, wines and oenological environment, spontaneous MLF are usually unpredictable because of the stressful conditions and especially due to acidity [2]. The consequence of the mismanagement of
this step might lead to the depreciation of wine quality. To obtain a clone more tolerant to acidity, we undertaken to replicate O. oeni in a temporally varying environment (pH 5.3 to 3.2) to improve acid tolerance. To discriminate stress tolerance of evaluated populationsversus parental strain an ethanol or acid stress were improved to both population.
Domaines
Biochimie, Biologie MoléculaireOrigine | Fichiers produits par l'(les) auteur(s) |
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