Food contact materials (FCM) represent a major economic issue and a large field of innovation. Food packaging production must be in compliance with the European Regulation 1935/2004: the third article specifies that FCM must not transfer their constituents to food in quantities which could endanger human health under normal or foreseeable conditions of use. Indeed, these materials are not inert and, in addition to started substances, Non-Intentionally Added Substances (NIAS) are able to be released into the food. NIAS can be contaminants from recycled materials, impurities, synthetic residues, new substances formed along the packaging production chain… Then, NIAS could represent a larget part of migrating substances which are not risk assessed, some being unpredictabled (Grob et al., 2006 ; Skejevrak et al., 2005). The European Regulation 10/2011 on plastic materials, multilayers and articles intended to come into contact with food regulates NIAS. It requires that the notion of the risk engendered by a substance concerns the substance itself, the impurities of this substance and any reaction or degradation products that are foreseeable in the context of the intended use. However there are currently no available guidelines to assess the risk of NIAS. They may also be present in other FCM than plastics, such as paper and boards... Because of the complex nature of FCM extract, it is pertinent to evaluate the potential toxicity of FCM as a whole taking into account any « cocktail effect ». The objectives of this study are to provide to packaging and food companies scientific relevant tools combining physicochemical and toxicological strategies based on bioassays applied to FCM extracts, especially paper and boards. Four toxicological endpoints were checked as relevant for low dose exposure: cytotoxicity, genotoxicity , oxidative stress and endocrine disruption activities.