Bisphenol A disrupts the intestinal lipid metabolism
Résumé
Purpose: Study the effect of Bisphenol A on intestinal lipid metabolism in vivo and in vitro.
Methods: In vivo, C57Bl/6 mice were exposed to 0.2 μg/kg bw/d BPA in drinking water. Treatment began at gestational day 6 and continued in offspring up to 36-week old. Four weeks after weaning, mice from treated-dams were fed with a high fat diet. During the BPA exposure, body weight, fat body mass and energy expenditure were checked. At 30-week old, we performed a lipid load test in male offspring mice. Mice were force-fed with corn oil (0.5 ml) and the time-course changes in triglyceridemia was analysed.
In vitro, human colorectal adenocarcinoma caco-2 cells were exposed to BPA doses (10−5 to 10−15 M). Expression of genes involved in lipid metabolism, L-FABP, CD36, ERα and β, ERRα and γ were investigated. The mRNA levels were quantified by quantitative RT-PCR.
Results: Results showed a sex-dependent effect of BPA exposure. Body weight of male pups born to BPA-exposed dams was significantly higher (15–30%) compared with controls. It appears in positive correlation with adipose tissue and in negative correlation with O2 consumption and energy expenditure. The lipid load test indicated that plasma triglyceride levels were decreased in BPA-treated mice suggesting that BPA affects either lipid uptake or the intestinal lipoprotein processing. In vitro, BPA altered the mRNA expression of studied genes suggesting that the intestinal lipoprotein processing was activated.
Conclusion: These data suggest that BPA could facilitate the lipid storage and consequently the obesity.